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Comparison of primers for RAPD-PCR from environmental isolates of Cryptococcus neoformans, Cryptococcus albidus and Cryptococcus laurentii complex BJM
Pedroso,Reginaldo dos Santos; Ferreira,Joseane Cristina; Costa,Karen Regina Carim da; Candido,Regina Celia.
Various organisms have been characterized by molecular methods, including fungi of the genus Cryptococcus. The purposes of this study were: to determine the discriminatory potential of the RAPD (Random Amplified Polymorphic DNA) primers, the pattern of similarity of the Cryptococcus species, and discuss their useful application in epidemiological studies. We analyzed 10 isolates of each specie/group: C. albidus, C. laurentii complex, C. neoformans var. grubii, all from environmental source, and two ATCC strains, C. neoformans var. grubii ATCC 90112, and C. neoformans var. neoformans ATCC 28957 by RAPD-PCR using the primers CAV1, CAV2, ZAP19, ZAP20, OPB11 and SEQ6. The primers showed a good discriminatory power, revealing important differences between them...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptococcus albidus; Cryptococcus laurentii; RAPD; Molecular Markers.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300015
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Impact of a medication therapy management service offered to patients in treatment of breast cancer BJPS
Amaral,Pedro Augusto do; Mendonça,Simone de Araújo Medina; Oliveira,Djenane Ramalho de; Peloso,Leonardo José; Pedroso,Reginaldo dos Santos; Ribeiro,Maria Ângela.
ABSTRACT Cancer has high morbidity and mortality rates related to medication use and produce a costly impact in health care. Thus, patients require constant monitoring and proper coordination of care between different professionals. This study aimed to evaluate the impact generated by a Medication Therapy Management service (MTM) offered to patients with breast cancer in use of polypharmacy. Observational, exploratory, descriptive and retrospective study of a MTM service that included 93 patients. Sociodemographic and clinical data related to pharmacotherapy and the processes associated with the systematization of the service were collected and analyzed. Patients were followed-up by the MTM service on average for 18 months (±4.31) and 185 drug-related...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medication therapy management services; Outcomes Pharmaceutical care; Oncology; Breast Cancer..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200626
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